5-AMINO-1MQ

Research Benefits

• NNMT Inhibition — 5-Amino-1MQ is a small molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme implicated in obesity, metabolic syndrome, and cancer biology.
• Metabolic Activation — By inhibiting NNMT, 5-Amino-1MQ increases intracellular NAD+ and SAM levels, enhancing cellular energy metabolism and methylation capacity.
• Adipocyte Research — NNMT inhibition reverses diet-induced obesity in murine models by reducing fat cell size and inhibiting adipocyte differentiation.
• Energy Expenditure — Published studies show NNMT inhibition increases basal energy expenditure through enhanced mitochondrial uncoupling and thermogenesis.

Mechanism of Action

5-Amino-1MQ (5-Amino-1-methylquinolinium) is a cell-permeable, selective inhibitor of NNMT, an enzyme that methylates nicotinamide using SAM as the methyl donor.

NNMT Inhibition: By blocking NNMT, 5-Amino-1MQ prevents the methylation of nicotinamide to 1-methylnicotinamide (1-MNA). This has two metabolic consequences:

NAD+ Preservation: Nicotinamide is a precursor to NAD+ via the salvage pathway (NAMPT). When NNMT consumes nicotinamide, NAD+ synthesis is reduced. NNMT inhibition preserves the nicotinamide pool, increasing NAD+ availability for sirtuins, PARPs, and metabolic enzymes.

SAM Conservation: NNMT consumes SAM (S-adenosylmethionine) as a methyl donor. Inhibiting NNMT conserves SAM for other methylation reactions (DNA methylation, histone methylation, phospholipid synthesis), restoring epigenetic and metabolic methylation balance.

Anti-Adipogenic: In adipocytes, NNMT inhibition activates SIRT1 (via increased NAD+), which deacetylates PPARγ and C/EBPα, suppressing adipogenic gene programs and reducing lipid accumulation.

Research Protocol

For research use only.

Preparation

5-Amino-1MQ is water-soluble. Prepare stocks at 10-50 mM in sterile water or PBS.

Research Dosing (from published studies)

• In vitro: 10-100 μM for adipocyte differentiation inhibition and NNMT activity assays.
• In vivo (murine, HFD model): 10-50 mg/kg/day oral or IP for 10-14 days in diet-induced obesity studies.

Storage

Store powder at -20°C. Solutions at 2-8°C, use within 14 days.

Safety & Handling

Research Use Only.

Known Considerations

• 5-Amino-1MQ is a research-grade compound without extensive toxicological profiling.
• NNMT is expressed in many tissues; systemic inhibition may have effects beyond adipose tissue.
• Monitor hepatic function in chronic dosing studies, as NNMT plays a role in liver xenobiotic metabolism.

Frequently Asked Questions

What is 5-Amino-1MQ?

5-Amino-1MQ is a small molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that depletes both NAD+ precursors and SAM. Inhibiting NNMT increases cellular NAD+ and SAM, enhancing energy metabolism and methylation.

How does NNMT inhibition affect metabolism?

NNMT consumes nicotinamide (NAD+ precursor) and SAM (universal methyl donor). Blocking this enzyme preserves both metabolites, increasing NAD+-dependent sirtuin activity and SAM-dependent methylation, which collectively enhance energy expenditure and suppress adipogenesis.

What purity is this product?

BeaCapra 5-Amino-1MQ is ≥99% pure by HPLC with COA provided.